Insulin‐like growth factor (IGF‐I) mRNA and IGF‐I receptor in trout testis and in isolated spermatogenic and Sertoli cells
Identifieur interne : 003F51 ( Main/Exploration ); précédent : 003F50; suivant : 003F52Insulin‐like growth factor (IGF‐I) mRNA and IGF‐I receptor in trout testis and in isolated spermatogenic and Sertoli cells
Auteurs : Florence Le Gac [France] ; Maurice Loir [France] ; Pierre-Yves Le Bail [France] ; Martine Ollitrault [France]Source :
- Molecular Reproduction and Development [ 1040-452X ] ; 1996-05.
Abstract
Few data exist concerning the occurrence and potential role of an insulin‐like growth factor (IGF) system in fish gonads. Using Northern and slot blot hybridization with a specific salmon IGF‐I cDNA, we confirmed that IGF‐I transcription occurs in trout testis. Testicular IGF‐I mRNA abundance may be increased by long‐term GH treatment in juvenile fish, while shorter treatment with growth hormone (GH) or a gonadotropin (GTH‐II) in maturing males had no statistically significant effect. Radiolabelled recombinant human IGF‐I binds with high affinity to crude trout testis preparation, to cultured isolated testicular cells, and to a membrane fraction of these cells (Ka = 0.2 to 0.7 × 1010 M1; Bmax = 10 to 20 fmol/107 cells, and 68 fmol/mg protein of membrane). The binding site was identified as type 1 IGF receptor by its binding specificity (IGF‐I > IGF‐II ⋙ insulin) and the molecular size of its α‐subunit labelled with 125HGF‐I (Mr125 ‐ 140 kDa). 125HGF‐II also bound to the type 1 receptor whereas IGF‐II/mannose 6 phosphate receptors could not be detected. Separation of isolated testicular cells by Percoll gradient and centrifugal elutriation provided populations enriched in different types of intratubular cells. IGF‐I mRNA (detected by reverse transcription + polymerase chain reaction [PCR]) and IGF‐I receptors (measured by competitive binding) were observed to a greater extent in Sertoli cell‐enriched populations and in spermatogonia with primary spermatocytes. Therefore, IGF‐I is a potential paracrine/autocrine regulator inside the spermatogenic compartment and appears as a possible mediator of GH action at the gonadal level in fish. © 1996 Wiley‐Liss, Inc.
Url:
DOI: 10.1002/(SICI)1098-2795(199605)44:1<23::AID-MRD3>3.0.CO;2-V
Affiliations:
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Company</publisher><pubPlace>Hoboken</pubPlace><date type="published" when="1996-05">1996-05</date></imprint><idno type="ISSN">1040-452X</idno></series></biblStruct></sourceDesc><seriesStmt><idno type="ISSN">1040-452X</idno></seriesStmt></fileDesc><profileDesc><textClass></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">Few data exist concerning the occurrence and potential role of an insulin‐like growth factor (IGF) system in fish gonads. Using Northern and slot blot hybridization with a specific salmon IGF‐I cDNA, we confirmed that IGF‐I transcription occurs in trout testis. Testicular IGF‐I mRNA abundance may be increased by long‐term GH treatment in juvenile fish, while shorter treatment with growth hormone (GH) or a gonadotropin (GTH‐II) in maturing males had no statistically significant effect. Radiolabelled recombinant human IGF‐I binds with high affinity to crude trout testis preparation, to cultured isolated testicular cells, and to a membrane fraction of these cells (Ka = 0.2 to 0.7 × 1010 M1; Bmax = 10 to 20 fmol/107 cells, and 68 fmol/mg protein of membrane). The binding site was identified as type 1 IGF receptor by its binding specificity (IGF‐I > IGF‐II ⋙ insulin) and the molecular size of its α‐subunit labelled with 125HGF‐I (Mr125 ‐ 140 kDa). 125HGF‐II also bound to the type 1 receptor whereas IGF‐II/mannose 6 phosphate receptors could not be detected. Separation of isolated testicular cells by Percoll gradient and centrifugal elutriation provided populations enriched in different types of intratubular cells. IGF‐I mRNA (detected by reverse transcription + polymerase chain reaction [PCR]) and IGF‐I receptors (measured by competitive binding) were observed to a greater extent in Sertoli cell‐enriched populations and in spermatogonia with primary spermatocytes. Therefore, IGF‐I is a potential paracrine/autocrine regulator inside the spermatogenic compartment and appears as a possible mediator of GH action at the gonadal level in fish. © 1996 Wiley‐Liss, Inc.</div></front></TEI><affiliations><list><country><li>France</li></country><region><li>Région Bretagne</li></region><settlement><li>Rennes</li></settlement></list><tree><country name="France"><region name="Région Bretagne"><name sortKey="Le Gac, Florence" sort="Le Gac, Florence" uniqKey="Le Gac F" first="Florence" last="Le Gac">Florence Le Gac</name></region><name sortKey="Le Bail, Pierre Ves" sort="Le Bail, Pierre Ves" uniqKey="Le Bail P" first="Pierre-Yves" last="Le Bail">Pierre-Yves Le Bail</name><name sortKey="Le Gac, Florence" sort="Le Gac, Florence" uniqKey="Le Gac F" first="Florence" last="Le Gac">Florence Le Gac</name><name sortKey="Loir, Maurice" sort="Loir, Maurice" uniqKey="Loir M" first="Maurice" last="Loir">Maurice Loir</name><name sortKey="Ollitrault, Martine" sort="Ollitrault, Martine" uniqKey="Ollitrault M" first="Martine" last="Ollitrault">Martine Ollitrault</name></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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